Query Sequence Data

Site(s) Click name for description

Inferred Identity(s)

Bechler-Ferris 1 Bonehole Fairy Fairy Run-Off Rods Hillside - Upper Hillside Enrichments Hillside Lower Hillside Neighbor Imperial Inkwell Joseph's Coat #27 Joseph's Coat #41 Ponuntpa Potts Practice Rabbit Creek Red Sentinel Spray Geyser West Thumb Western Pool Witch Pond Select all

Acidobacter Acidobacteria Acidobacterium Aquificae CFB CFB Group Candidate Division OP10 Candidate Division WS6 Candidate NKB19 Candidate OP10 Chlorobi Chloroflexi - Chemotrophic Chloroflexi - Chemotropic Chloroflexi - Green Chloroflexi - Red Cyanobacteria Deinococcus/Thermus Firmicutes Gram Positive Green Nonsulfur Green Sulfur Nitrospira Planctomyces Planctomycetes Proteobacteria Proteobacteria - Alpha Proteobacteria - Delta Proteobacteria - Gamma Spirochete Unknown Artesian Basin Unknown Octopus Spring Verrucomicrobia unknown Select all

Forward Primer:	Select... 77GNS (Boomer et al., 2002) 8FPL (Reysenbach et al., 1994) CCR344F (Nubel et. al, 2001)
Reverse Primer:	Select... 1492RPL (Reysenbach et al., 1994) 953RRED (Boomer et al., 2002) CCR1338R (Nubel et. al, 2001)
DNA Methods:	Select... Alkaline Lysis (Epicentre MasterPure Kit) Bead Beater/Detergent, Phenol/Chloroform (Boomer et al. 2002) Lysozyme/Detergent, Phenol/Chloroform (Boomer et al. 2002) Lysozyme/Detergent, Phenol/Chloroform (Boomer et al. 2002)Lysozyme/Detergent, Phenol/Chloroform (Boomer et al. 2002)
Sources:	Select... Mat - green layer Mat - red layer Red Enrichment Red Enrichment Red Enricment Red Layer Red Layer Rod Rod - green layer Rod - red layer Sourcewater mat - orange puffs mat - red layer mat - red layer red layer sourcewater tree branch

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Send research questions to: Dr. Sarah Boomer (boomers@wou.edu)